Krakatoa-HC CB23726HC (1000U)
HTA-Protease© with E,L,F preferred cleavage sites. Optimal activity at pH 3.0, 80°C.
Reference #: | CB23726HC |
| Class: | HTA-Protease |
| Optimal pH: | 3.0 |
| pH Range: | 1.8-4.2 |
| Optimal Temp: | 80°C |
| Temp. range: | 40-100°C |
| Shelf Life: | >2 years (ambient) |
Description
Krakatoa-HC© is shipped ready-to-use, requires no aliquoting, and is stored ambiently. Krakatoa cuts at E, L, and F residues preferentially. No autolysis is observed when using Krakatoa on its own or when combined with Vesuvius HTA-Protease©. HTA-Protease digestions should be conducted at 70-90°C and pH of 2-4 for 5-90 minutes and do not require chaotropes, sample manipulation, or carbamidomethylation. Using brief one-step HTA-Protease reaction protocols significantly increase proteome and protein sequence coverage with datasets orthogonal to trypsin and have shown a bias for ribonucleoproteins, histones, mitochondrial, and membrane proteins.
Product Documentation
Enzyme storage
DO NOT FREEZE. Store enzymes at ambient temperatures. Enzyme preparations are stored in aqueous buffered solutions at pH 3 with 20mM potassium phosphate dibasic and 40mM citric acid. Optimal shelf lives are observed in this buffer at ambient temperatures. Because of this storage condition, reasonable care should be taken to avoid microbial contamination of the enzyme aliquot.
Materials Included
Each order includes one 1000U tube of Krakatoa-HC and one 1mL tube of 10x HTA-Protease buffer.
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Certificates of Analysis
Lot Number:
